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SEMICRITICAL-medical devices that touch mucous membranes or skin that is not intact require a disinfection process (high-level disinfection [HLD]) that killsSterilization: Article, surface, or medium is freed of all living Disinfection: Destruction of microorganisms, especially potential. Cold chemical sterilization is a common and inex- pensive method of sterilizing items that cannot be exposed to steam sterilization. The most common chemical Sterilization and Disinfection. Sterilization is defined as the process where all the living microorganisms, including bacterial spores are killed. Within the range of sterilizing temperatures, the time required to produce a lethal effect is inversely proportional to temperature employed. Eg.,. Dry heat at TWO METHODS:- 1. Physical method a)Dry heat sterilization: Eg: Incineration, Direct flame, Red heat, Hot air. b)Moist heat sterilization:. Overloading alters heat convection and increases the time required to sterilize. Source: Perkins 1983. Note: High-speed (flash) prevacuum sterilizers are.