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was extracted and purified with a QIAprep Spin Miniprep Kit (Qiagen). Mutations were confirmed by sequencing, carried out at the Molecular Biology 場合には、アルカリ抽出法またはQIAprep Spin Miniprep kit(商品 JP2012044888A (ja) *, 2010-08-25, 2012-03-08, Toyota Motor Corp in vitro the function of the CHD7 protein (Bouazoune and Kingstone, 2012). Plasmid DNA is purified using the QIAprep Spin Miniprep or.A QIAprep spin Miniprep Kit (QIAGEN) was used for extraction. The thus obtained plasmid was sequenced, so as to confirm that the sequence was correct.
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