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This guide explains how to denature and dilute prepared libraries for sequencing on the. Illumina® NextSeq® system. This guide includes instructions forInstructions for denaturing and diluting libraries before sequencing on the MiSeq System. This guide explains how to denature and dilute prepared libraries for sequencing on the Illumina®. NextSeq™ system. This guide includes instructions for This guide explains steps to denature and dilute libraries after sample with the PhiX control prior to loading them onto the MiSeq reagent cartridge. After denaturing and diluting your libraries and preparing the optional PhiX control, you are ready to load libraries onto the reagent cartridge and set up MiSeq System Denature and Dilute Libraries Guide. (document # 15039740). Provides instructions for denaturing and diluting prepared sample libraries before. Denature a 2 nM Library 1 Combine the following volumes in a microcentrifuge tube. 2 Vortex briefly and then centrifuge at 280 × g for 1 minute. 3 Incubate This guide explains how to denature and dilute prepared libraries for sequencing on the. Illumina® MiniSeq™ system. This guide includes instructions for
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