Bd facscalibur manual

BD Biosciences 2350 Qume Drive San Jose, CA 95131-1807 USA Tel (877) 232-8995 Fax (408) 954-2347 Brazil Tel (55) 11-5185-9995 Fax (55) 11-5185-9895 magnetic, optical, chemical, manual, or otherwise, without prior written permission from BD Biosciences. The information in this guide is subject to change without notice. BD Biosciences Video called 'BD FACSCalibur Start-up and Clean-up Procedures', tagged as cytometer cleaning procedure, cytometer start up, cytometer shut down. Our BD FACSCalibur is a one laser, five detector analysis flow cytometer capable of measuring 3 colors simultaneously. It is equipped with a "Blue" (488nm) laser and a DDM board and data collected include forward scatter, side scatter, FL1 (530±30nm), FL2 (585±42nm), and FL3 (650±30nm). The acquisition workstations is a Mac G4 running BD The BD FACSCalibur Flow Cytometer is an innovative platform used for both cell analysis and cell sorting in a single benchtop unit. The system is designed with a multicolor application support which allows accommodation of a wide range of laboratory and clinical applications. Engineered to improve laboratory productivity, the BD FACSCalibur BD FACSDiva software features specific to the BD FACSCanto cytometer within this manual. BD Biosciences recommends that first-time user's of this instrument take advantage of operator training offered with the sale of every new instrument. The BD FACSCanto Flow Cytometer Reference Manual assumes you have a working knowledge of basic Microsoft (BD) FACSVantage-SE high speed cell sorter, and a BD FACSCalibur bench top flow cytometer. The FACSVantage-SE will sort or isolate subpopulations of cells from a As mentioned previously, aspects of this project are conducted in a manual fashion in the laboratories listed, and are therefore authorized by the individual PI‟s BUAs. Dilute the sample in the same fluid as sheath. If you are running BD Calibrite beads, dilute them in BD FACSFlow solution and use BD FACSFlow for sheath. Acquisition Troubleshooting (continued) Chapter 9: Troubleshooting 195 BD FACSComp Troubleshooting. PMT Adjustment. Observation Possible Causes Recommended Solutions User Manual FEATURES The BD FACSCalibur was the first system to provide standard multicolor analysis capabilities by incorporating an alignment free optical design, interbeam compensation, and dual-laser technology. Dual laser design for multicolor analysis. own reference manual, provided on the documentation CD. BD Biosciences recommends that first-time users of this instrument take advantage of operator training offered with the sale of every new instrument. The BD FACSCanto II Flow Cytometer Reference Manual assumes you have a working knowledge of basic Microsoft® Windows® operation. If you This user's guide contains the instructions necessary to operate and maintain your BD FACSAria™ flow cytometer. Because many instrument functions are controlled by BD FACSDiVa™ software, this guide also contains basic software information needed for instrument setup. For more detailed instructions on how to use the software, refer to the